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Breaking-Cas

Oligo guide design tool for CRISPR based genome editing.


Breaking Cas server is maintained since 2018 by Juan Antonio García-Martín
 
Please cite:
"Juan C. Oliveros, Mònica Franch, Daniel Tabas-Madrid, David San-León, Lluis Montoliu, Pilar Cubas and Florencio Pazos (2016). Breaking-Cas––interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes. Nucleic Acids Research (2016) doi: 10.1093/nar/gkw407. https://bioinfogp.cnb.csic.es/tools/breakingcas"

Tutorial

1 Choose organism: (alphabetic list) Write 3 letters or more and select it.
2 Paste one or several query DNA sequences in FASTA format (up to 20,000 nucleotides in total):
Or upload FASTA file (DNA): 
3 Select nuclease settings:
Or set your own parameters:
PAM sequence: Use predefined settings for Cas9 or Cpf1, or set custom parameters for other nucleases. If necessary, write a different PAM sequence (in IUPAC notation). For Cas9, positional weights based on Hsu et al. (2013) are used by default. See tutorial pages for details on off-targets score's calculation.
PAM position: 5' 3'
Guide length:
Mismatches:
pepe
 
Confirmation email (optional): 		 
To receive a message as soon the job finishes. Write it carefully (it will not be checked).

Fill with example              Clear fields
BioinfoGP, Centro Nacional de Biotecnología (CNB-CSIC)