Basic statistics
| Feature | R1 | R2 |
|---|---|---|
| File Name | 1M_SRR9336476_1.fastq.gz | 1M_SRR9336476_2.fastq.gz |
| Number Of Reads | 1,000,000 | 1,000,000 |
| Sample Size | 1,000,000 | 1,000,000 |
| Average Read Length | 150nt | 150nt |
| GC content | 0.42 | 0.42 |
| Adapter Sequence | AGATCGGAAGAGCACACGTCTGAACTCCAGTCA | AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT |
| Adapter Name | Illumina TruSeq Adapter Read 1 | Illumina TruSeq Adapter Read 2 |
Per base sequence quality boxplot
Read 1
Read 2
Per base sequence content
Read 1
Read 2
Sequence Duplication Levels
Read 1
Read 2
Overrepresented sequences
Read 1
| SEQUENCE | %READS |
|---|---|
| TCTTGGTAAGTAGCAATCTTCTTACCATCGACAATGATGTGCTTGTCATCGTGGGAAACTTCACCAGCGTATCTACCGTGAGTGGAGTCGTACTTGAACA | 0.21% |
| CAAGGTAGTGTAAGCGTCATCGGTGTAAGTCATGACTTGTTGATACACACTCAAGACATCAGATGGAATTTGGAATCCACTGTTGCCAGTTTCTAGACCC | 0.14% |
| CAATTACATAAAGAGAAGGTATAATAATGGAGGTAATTTTTCAAATATTTGTTGTAAAAGGTTCCCTTTTATAACAATAAAGCAGCTGCACCTGCAACAA | 0.14% |
| GGAGCGGCAGAGGATGTAGCTTCGGAAGAAGATGCAGCCTTGGAAGAAGATGCAGCCTCGGAAGAAGATGCAGCCTCGGAAGAAGCTGGGGAAACGGAGG | 0.13% |
| GGAAATATAACAGAGACAGAATATTTAATTTTATGTATGTAAGCATCGATTGGACACCAGGCTTATTGATGACCTTACTCGTCCAATTTGGCACGGACCG | 0.11% |
| GGAGCGACAGAAGAAGAAACGGCCTTAGAAGATGATTCGGCAGATGATGATGGGGCAGCAGAAGAAGATGGGGCAGCAGAAGAGGATGGAGCGGCAGAGG | 0.11% |
| GGAAGATGGAGCAGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAGCAGTGTCTAATTCCTTGAAAACACCAGTGGAGTCAATGGCGATG | 0.10% |
Read 2
| SEQUENCE | %READS |
|---|---|
| CCATTGCCTCTTTGGCCGTCGCTGACACCAGCGCCGCCGAAACTGCTGAATTGCAAGCTATTATCGGTGACATCAACTCTCATCTTTCTGACTACTTGGG | 0.29% |
| GCTTCTGCCGCTGCTAACGTTACCACTGCTACTGTCAGCCAAGAATCTACCACTTTGGTCACCATCACTTCTTGTGAAGACCACGTCTGTTCTGAAACTG | 0.15% |
| CGCTCTATTAAGATGAAATTATCAACTGTCCTATTATCTGCCGGTTTAGCCTCGACTACTTTGGCCCAATTTTCCAACAGTACATCTGCTTCTTCCACCG | 0.13% |
| CATAAACAAACAAAATGGTTAGAGTTGCTATTAACGGTTTCGGTAGAATCGGTAGATTGGTCATGAGAATTGCTTTGTCTAGACCAAACGTCGAAGTTGT | 0.13% |
| CGCCCAAAGTACTAGATTAGCCGCCGCCGTCTCTTCTTCAGCCGCCTCCGCGGGTGTCTCAAGGATAGCCGCTAGCGCCATGGCCAGCGCTATTTTCAAG | 0.11% |
| GTTTGATTGTCGTTGTTGTCATCTACATCATCGGTATTATTATTCAAATTGCATCTATCAACAAATGGTACCAATATTTCATCGGTAGAATTATTTCCGG | 0.10% |
| ACGGTTACGTTTTCATGGGCTGTTTGGTCTTCATGTTCTTCTATGTTTTGTTAGTTGTTCCAGAAACTAAGGGTTTGACTTTGGAAGAAGTCAACACCAT | 0.10% |
Adapter content
Read 1
| SEQUENCE | CONTENT |
|---|---|
| A | 0.05% |
| AG | 0.05% |
| AGA | 0.05% |
| AGAT | 0.04% |
| AGATC | 0.08% |
| AGATCG | 0.05% |
| AGATCGG | 0.04% |
| AGATCGGA | 0.04% |
| AGATCGGAA | 0.03% |
| AGATCGGAAG | 0.03% |
| AGATCGGAAGA | 0.03% |
| AGATCGGAAGAG | 0.03% |
| AGATCGGAAGAGC | 0.03% |
| AGATCGGAAGAGCA | 0.03% |
| AGATCGGAAGAGCAC | 0.03% |
| AGATCGGAAGAGCACA | 0.03% |
| AGATCGGAAGAGCACAC | 0.02% |
| AGATCGGAAGAGCACACG | 0.02% |
| AGATCGGAAGAGCACACGT | 0.02% |
| AGATCGGAAGAGCACACGTC | 0.02% |
| AGATCGGAAGAGCACACGTCT | 0.02% |
| AGATCGGAAGAGCACACGTCTG | 0.02% |
| AGATCGGAAGAGCACACGTCTGA | 0.02% |
| AGATCGGAAGAGCACACGTCTGAA | 0.02% |
| AGATCGGAAGAGCACACGTCTGAAC | 0.02% |
| AGATCGGAAGAGCACACGTCTGAACT | 0.01% |
| AGATCGGAAGAGCACACGTCTGAACTC | 0.01% |
| AGATCGGAAGAGCACACGTCTGAACTCC | 0.01% |
| AGATCGGAAGAGCACACGTCTGAACTCCA | 0.01% |
| AGATCGGAAGAGCACACGTCTGAACTCCAG | 0.01% |
| others | 0.28% |
Read 2
| SEQUENCE | CONTENT |
|---|---|
| A | 0.05% |
| AG | 0.05% |
| AGA | 0.05% |
| AGAT | 0.04% |
| AGATC | 0.08% |
| AGATCG | 0.05% |
| AGATCGG | 0.04% |
| AGATCGGA | 0.04% |
| AGATCGGAA | 0.03% |
| AGATCGGAAG | 0.03% |
| AGATCGGAAGA | 0.03% |
| AGATCGGAAGAG | 0.03% |
| AGATCGGAAGAGC | 0.03% |
| AGATCGGAAGAGCG | 0.03% |
| AGATCGGAAGAGCGT | 0.02% |
| AGATCGGAAGAGCGTC | 0.02% |
| AGATCGGAAGAGCGTCG | 0.02% |
| AGATCGGAAGAGCGTCGT | 0.02% |
| AGATCGGAAGAGCGTCGTG | 0.02% |
| AGATCGGAAGAGCGTCGTGT | 0.02% |
| AGATCGGAAGAGCGTCGTGTA | 0.02% |
| AGATCGGAAGAGCGTCGTGTAG | 0.02% |
| AGATCGGAAGAGCGTCGTGTAGG | 0.02% |
| AGATCGGAAGAGCGTCGTGTAGGG | 0.02% |
| AGATCGGAAGAGCGTCGTGTAGGGA | 0.01% |
| AGATCGGAAGAGCGTCGTGTAGGGAA | 0.01% |
| AGATCGGAAGAGCGTCGTGTAGGGAAA | 0.01% |
| AGATCGGAAGAGCGTCGTGTAGGGAAAG | 0.01% |
| AGATCGGAAGAGCGTCGTGTAGGGAAAGA | 0.01% |
| others | 0.34% |
Insert size estimation
