######################################################### # # # Useful info (AprendeBioinformáticaEnCasa: session 7) # # By: Juan Carlos Oliveros (BioinfoGP, CNB-CSIC) # # # ######################################################### # FIESTA viewer: to filter RNA-seq tables and view the results in # real time: https://bioinfogp.cnb.csic.es/tools/FIESTA/index.php # Generic URL for yeast genes to be used with FIESTA viewer: http://www.ensembl.org/Saccharomyces_cerevisiae/Gene/Summary?g=##ACCESSION## # Venny: tool to compare up to 4 gene lists (of other elements) # with Venn diagrams: https://bioinfogp.cnb.csic.es/tools/venny/ # Gene Ontology main page: controlled vocabulary of biological terms # and a popular tool to perform functional enrichment analysis http://geneontology.org/ # Enrichr (and modEnrichr): useful tool to perform quick functional # enrichment analysis using several databases of annotations # (available for a small number of model organisms) https://amp.pharm.mssm.edu/modEnrichr/ ################################ # # # GSEA (Broad Institute) tips # # (Preranked analysis) # # # ################################ -Use your own compendium of functional groups in gmt format (MyGMT is a tool to convert biomart tables containing GO terms into gmt files to be used with GSEA tool) https://bioinfogp.cnb.csic.es/tools/mygmt/ -Adjust "Basic fields": "Max size: exclude larger sets" and "Min size: exclude smaller sets" to use more or less functional groups as defined in the gmt file. -To reproduce a given analysis, copy the "seed for permutation" value at the results and copy it in "Advanced fields" Juan Carlos Oliveros 2020 BioinfoGP CNB-CSIC